Figure 4.
Time profiles of cell growth, S- or R- mandelic acid production, and residual glucose and acetic acid accumulation during the culture of the engineered E. coli. (A) OD600, S-mandelic acid produced, and (B) glucose consumed, byproduct acetic acid accumulated were monitored for BCAE with pSUFAAQ. (C) OD600, R-mandelic acid produced, and (D) glucose consumed, byproduct acetic acid accumulated were monitored for BCAE with pSUFAAQSD. Data shown are means ± standard deviations calculated from triplicate individual experiments.