Table 2.

Cocktails for DC maturation

Cocktail	Inflammatory Cytokines/IFN	Other Additives	TLR Ligands
C	TNF-α, IL-1β, IFN-γ	PGE2	CL075
CP	TNF-α, IL-1β, IFN-γ	PGE2	CL075, poly(I:C)
R	TNF-α, IL-1β, IFN-γ	PGE2	R848
RP	TNF-α, IL-1β, IFN-γ	PGE2	R848, poly(I:C)

Following concentrations were used in the individual maturation cocktails:

All cocktails contained 1100 IU/mL TNF-α, 2000 IU/mL IL-1β, 5000 U/ml IFN-γ and 250 ng/ml PGE₂.

Additional additives as follows:

Cocktail C: 1 μg/ml CL075

Cocktail CP: 1 μg/ml CL075 and 20 ng/ml poly (I:C)

Cocktail R: 1 μg/ml R848

Cocktail RP: 1 μg/ml R848 and 20 ng/ml poly (I:C)