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. 2011 Aug 26;8:106. doi: 10.1186/1742-2094-8-106

Figure 4.

Figure 4

Involvement of the PI3K/Akt pathway in TNF-α-induced MMP-9 release from pericytes. A, Effect of PI3K inhibitor (LY294002) on TNF-α-induced MMP-9 release from pericytes. Cells were pretreated with LY294002 for 15 min, and then incubated with TNF-α (100 ng/mL) for 24 h. MMP-9 levels in the culture media of pericytes were assayed by western blot. Band intensities were quantified by scanning densitometry and the data are expressed as percentage of control values (vehicle-treated pericytes). Each bar indicates mean ± S.E.M. for three experiments. *** P < 0.001, significantly different from vehicle-treated pericytes. #P < 0.05, significantly different from TNF-α-treated pericytes. B, Representative images showing western blots of total and phosphorylated Akt in the cell lysates of pericytes treated with TNF-α (100 ng/mL) for 24 h.