Figure 7.

Levels of nitric oxide and inflammatory molecules in BV2-conditioned media. The levels of nitric oxide (NO) and inflammatory molecules in BV2-conditioned media prepared similarly to the conditioned media used for indirect toxicity experiments, were assayed using Griess reagent and flow cytometry, respectively. Oligomeric Aβ (ADDL) increased levels of NO (n = 4 in all groups), TNF-α, IL-6 and MCP-1 (n = 8 in all groups) in BV2-conditioned medium, but sinomenine reduced the levels of these molecules. Asterisks denote significant differences (p < 0.05).