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Induction profile (black squares and curve) of a population evolved for approximately 700 generations in the presence of 15 μM IPTG and 350 μM Pgal. Also shown is the wild-type induction profile without Pgal (grey data points and fit), as well as the predicted profile for wild-type incorporating Pgal anti-induction (grey curve shifted to the right). The expression levels at 15 μM IPTG for wild-type (with anti-induction) and evolved strain are very similar.
