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. 2011 Sep 29;7(9):e1002266. doi: 10.1371/journal.ppat.1002266

Figure 2. Tri6 binds to its cognate promoter in vivo.

Figure 2

ChIP was performed with HA antibodies (HA-Ab) with the Tri6-HA complemented (Tri6-HA) and Tri6 mutant (tri6Δstrains. PCR was performed on the ChIP samples using the primers FGSG_03536 Promo F and FGSG_03536 Promo R, encompassing the 1.2 Kb upstream of the Tri6 ORF, using different amounts of input DNA in the PCR reaction. Input DNA: Lane 1 = 25 ng, Lane 2  = 2.5 ng and Lane 3  = 0.25 ng of DNA. Fusarium genomic DNA (Genomic) was used as control to monitor the size of the PCR fragment (arrow). This is representative of two independent experiments.