Table 2.
Target region | Primer sequence (5'-3') | |
---|---|---|
Adh | Long partial region | Adh1-F1:ATGTCTAATACTGCTGGTCA Adh1-F2: TGATGTTTACTTCTGGGAGG Adh1-R1: GATTGAATTGTGTTCTTTA Adh1-R2: TGTGGATTATGCAACGAAGA Adh2-F: TGTTGACTTCTGGGATGCCAA Adh2-R: ATGCTAACGATGCACCGCAA |
Internal primer | Adh1-F5: AGGAGAATGCAAGGACTGCGCT Adh2-F5: CATTGCAAGTCTGAGGAAAG |
|
reduced Adh1 | Adh1-F2 Adh1-R3: CAAAATGGTAGATAGGCTT |
|
Specific primers | spAdh1-F: TCTACCATTTTGTTGGGACT spAdh1-R: AACGCTTCCTGTACATTCAA |
|
spAdh2-F: GATTAATCACTTCCTCGGCA spAdh2-R: TAATATAGCCGGTGCACTCT |
||
LEAFY | Long partial | LFY-F: TGTCGGAGGAGCCAGTGCAA LFY-R: GGCGTAGCAGTGCACATAGT |
LFY1int2 | LFY1-F: TGGACGTTCATCAATAAAGA LFY1-R: AGTCGAACTAAATAGTTGAA |
|
LFY2int2 | LFY2-F: GTGGGCCCATTTCCTGTAGT LFY2-R: GTTAAATCCGGTCAGATTAT |
|
LFY2s | LFY2S-F: CTGTATTGACTATTTCTGTC | |
MLFY2-F: CGTACGCTTATTTCTACTGCA LFY2-R |
||
Actin | Partial | Pact-F: CCATCCAGGCTGTTCTCTC Pact-R: GCAAGGTCCAGACGAAGG |