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. 2011 Sep 14;11:255. doi: 10.1186/1471-2148-11-255

Table 2.

Primers used for PCR amplification and sequencing in this study

Target region Primer sequence (5'-3')
Adh Long partial region Adh1-F1:ATGTCTAATACTGCTGGTCA
Adh1-F2: TGATGTTTACTTCTGGGAGG
Adh1-R1: GATTGAATTGTGTTCTTTA
Adh1-R2: TGTGGATTATGCAACGAAGA
Adh2-F: TGTTGACTTCTGGGATGCCAA
Adh2-R: ATGCTAACGATGCACCGCAA
Internal primer Adh1-F5: AGGAGAATGCAAGGACTGCGCT
Adh2-F5: CATTGCAAGTCTGAGGAAAG
reduced Adh1 Adh1-F2
Adh1-R3: CAAAATGGTAGATAGGCTT
Specific primers spAdh1-F: TCTACCATTTTGTTGGGACT
spAdh1-R: AACGCTTCCTGTACATTCAA
spAdh2-F: GATTAATCACTTCCTCGGCA
spAdh2-R: TAATATAGCCGGTGCACTCT

LEAFY Long partial LFY-F: TGTCGGAGGAGCCAGTGCAA
LFY-R: GGCGTAGCAGTGCACATAGT
LFY1int2 LFY1-F: TGGACGTTCATCAATAAAGA
LFY1-R: AGTCGAACTAAATAGTTGAA
LFY2int2 LFY2-F: GTGGGCCCATTTCCTGTAGT
LFY2-R: GTTAAATCCGGTCAGATTAT
LFY2s LFY2S-F: CTGTATTGACTATTTCTGTC

MLFY2-F: CGTACGCTTATTTCTACTGCA
LFY2-R

Actin Partial Pact-F: CCATCCAGGCTGTTCTCTC
Pact-R: GCAAGGTCCAGACGAAGG