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. 2011 Sep 19;8:74. doi: 10.1186/1742-4690-8-74

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Copyright ©2011 Clerc et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cellular localization of WT ASP and ASP-mut66 in transfected Jurkat T cells. Jurkat cells transfected with pcDNA-Flag-ASP (A-C) or pcDNA-Flag-ASPmut66 (D) were layered on glass slides, fixed, permeabilized, and stained with fluorescence-labelled antibodies as described in Figure. 3. The morphology of the cell was assessed by Normaski differential interference contrast (NDIC). White bars correspond to a scale of 10 μm. (E) Percentage of the total transfected cells with ASP showing an unpolarized distribution (white bar), a polarized location (grey bar), or a localization into membrane protrusion (hashed bar). A total of 206 cells from three separate experiments were scored.