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ASP localization in membrane connection. Jurkat cells transfected with pcDNA-Flag-ASP (A-C) or pEGFP (D) were layered on glass slides (A) or seeded at high density on polylysine-covered glass slides (B-D). The localization of ASP was analyzed as described above. The morphology of the cell was assessed by NDIC. White bars correspond to a scale of 10 μm.
