Table 3. Tumor-free time period: Hazard ratios (HR) for low versus high levels of immune markers.

Models†			Unadjusted model				Adjusted
Model	N	Comparison	HR	95% CI	p-value	Age*	HR	95% CI	p-value
CD3ε	136	low vs. high	1.9	1.1–3.2	0.03	50	2.6	1.2–5.7	0.01
						60	1.8	1.03–3.2	0.04
						70	1.2	0.63–2.4	0.53
						Age x CD3ε interaction p = 0.10
IFN-γ	138	low vs. high	0.71	0.42–1.2	0.21	any	0.73	0.42–1.2	0.24
CD25	136	low vs. high	1.9	1.1–3.3	0.02	50	3.2	1.4–7.2	0.004
						60	1.9	1.04–3.3	0.04
						70	1.1	0.55–2.1	0.84
						Age x CD25 interaction p = 0.02
CD68	137	low vs. high	2.3	1.3–4.2	0.004	50	3.9	1.7–8.7	0.001
						60	2.3	1.29–4.2	0.005
						70	1.4	0.70–2.8	0.34
						Age x CD68 interaction p = 0.04
ICAM-1	136	low vs. high	1.6	0.94–2.8	0.08	50	2.3	1.1–4.9	0.04
						60	1.6	0.91–2.8	0.11
						70	1.1	0.57–2.2	0.75
						Age x ICAM-1 interaction p = 0.12
IL-10	79	low vs. high	1.6	0.82–3.2	0.16	any	1.8	0.87–3.7	0.12
Score**	135	1–3 vs. 0	2.8	1.3–5.9	0.008	50	4.5	1.5–14	0.009
						60	2.6	1.2–6	0.02
						70	1.5	0.6–3.5	0.38
		4 vs. 0	2.5	1.2–5.4	0.02	50	4.2	1.4–12.9	0.01
						60	2.4	1.09–5.5	0.03
						70	1.4	0.60–3.4	0.42

Separate Cox models for each biomarker.

^†Adjusted for gender, age at initial BCC, interaction of age with time.

*Some models have an interaction of biomarker level (binary) with age at initial BCC removal (continuous variable), as shown above. When the interaction was present, the effect of mRNA marker level is interpreted for a specific age.

**Combined score defined as the number of mRNA markers out of CD3ε, CD25, CD68, and ICAM-1 that are below the median: 0 (none below) / 1–3 (some below) / 4 (all below). P-value for age by score interactions are p = 0.07 and p = 0.08 for some vs. none and all vs. none, respectively.

Effects are presented separately for age 50, 60, and 70 where age moderated effects.