FIGURE 5:

The loss of TRS65 exaggerates the Snc1p recycling defect and the COPI localization defect in the gea1-19gea2Δ mutant. (A) Yeast cells expressing GFP-Snc1p or Sec21p-GFP were grown in YPD media at 25°C to early log phase and pelleted. Two OD₆₀₀ units of cells were harvested, shifted to prewarmed media at 37°C, and incubated for 5 min before the cells were directly examined by fluorescence microscopy. Scale bar, 3 μm. (B) Quantitation of the data in A. The data shown are representative. Approximately 34–46 cells were quantitated.