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. 2011 Oct 1;22(19):3634–3644. doi: 10.1091/mbc.E11-03-0197

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© 2011 Chen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — The loss of Trs65p exaggerates the COPI localization defect in the trs120-2 and trs120-4 mutants. (A) Yeast cells expressing Sec21p-GFP, GFP-Vrg4p or Sec7p-GFP were grown to early log phase in YPD medium at 25°C. A total of 0.4 OD₆₀₀ unit of cells was harvested, resuspended in ice-cold YPD media, and directly examined by fluorescence microscopy. Scale bar, 3 μm. (B) Quantitation of data in A. The data shown are representative. For the trs120-2 and trs120-4 single and double mutants, ∼114–285 cells were quantitated.