TABLE 1:
Relocalization of Psy1 in various mutants.
| Frequency of localization pattern of GFP-Psy1 (%) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 25°C, 2 d | 25°C, 16 h; 34°C, 5 h | |||||||||
| PM | agg | PM + agg | PM + FSM | FSM | PM | agg | PM + agg | PM + FSM | FSM | |
| Wild type | 6.4 | 0 | 0 | 0 | 93.6 | 24.9 | 1.3 | 0.3 | 0 | 73.5 |
| myo1Δ | 9.2 | 0 | 85.5 | 5.3 | 0 | nd | nd | nd | nd | nd |
| fim1Δ | 9.6 | 0 | 86.1 | 4.3 | 0 | nd | nd | nd | nd | nd |
| end4Δ | 9.5 | 0 | 0.9 | 7.2 | 82.4 | 12.5 | 0 | 31.0 | 19.0 | 37.5 |
| cps8-188 | 12.3 | 0 | 0 | 1.2 | 86.5 | 16.5 | 0.4 | 31.9 | 6.2 | 45.1 |
Wild-type (YN68), myo1Δ (ZK194), fim1Δ (ZK186), and end4Δ (ZK69) strains expressing GFP-Psy1 were incubated on SSA at 25C for 2 d or 16 h and then shifted to 34C for 5 h. For each sample, >200 cells were counted. Data shown are from a single representative experiment of three carried out with similar results. agg, aggregate; nd, not determined.