Table 3. Specificity constants for human wild-type and nucleotide-binding mutant c-NADP-ME variants.

c-NADP-ME	k cat,NADP /K m,NADP*K m,mal(NADP) (s−1 mM−2)			Fold decrease in k cat,NADP /K m,NADP*K m,mal(NADP)	k cat,NAD /K m,NAD*K m,mal(NAD) (s−1 mM−2)			Fold increase in k cat,NAD /K m,NAD*K m,mal(NAD)
WT	32809	±	6633	1	0.5	±	0.1	1
E314A	137625	±	39042	0.2	19	±	5.3	35
S346K	12404	±	1843	2.6	0.3	±	0.04	0.5
E314A/S346K	42947	±	5948	0.8	6.2	±	1.0	12
K347Y	2733	±	173	12	0.7	±	0.2	1.3
K362Q	115	±	9.1	2.9×102	0.9	±	0.2	1.7
K362H	117	±	11.4	2.8×102	1	±	0.2	1.8
S346K/K347Y	810	±	147	41	0.7	±	0.1	1.3
S346K/K362Q	6	±	0.71	5.8×103	2.1	±	0.4	3.9
K347Y/K362Q	1.3	±	0.070	2.5×104	0.3	±	0.04	0.6
S346K/K347Y/K362Q	0.1	±	0.017	3.4×105	3.4	±	0.4	6.4
E314A/S346K/K347Y/K362Q	2.3	±	0.9	1.5×104	73	±	15	1.4×102
S346K/K347Y/K362H	0.03	±	0.011	1.2×106	5.3	±	0.9	10
E314A/S346K/K347Y/K362H	1.3	±	0.22	2.5×104	239	±	66	4.5×102
S346I/K347D/K362H	3.5×10−4	±	3.1×10−4	9.4×107	3.7	±	0.5	6.8
E314A/S346I/K347D/K362H	0.02	±	0.010	1.1×106	59	±	11.8	1.1×102