Table 4. Residues involved in ATP inhibition for human wild-type and nucleotide-binding mutant c-NADP-ME variants.

c-NADP-ME	Residue				aATP inhibition (%)
	314	346	347	362	bNAD+	bNADP+
WT	E	S	K	K	87.3	99.4
E314A	A	S	K	K	47.3	96.6
S346K	E	K	K	K	83.8	96.8
E314A/S346K	A	K	K	K	33.2	99.3
K347Y	E	S	Y	K	94.2	96.6
K362Q	E	S	K	Q	95.4	99.8
K362H	E	S	K	H	88.0	89.3
S346K/K347Y	E	K	Y	K	75.2	86.7
S346K/K362Q	E	K	K	Q	86.5	91.8
K347Y/K362Q	E	S	Y	Q	99.2	91.6
S346K/K347Y/K362Q	E	K	Y	Q	71.4	81.3
E314A/S346K/K347Y/K362Q	A	K	Y	Q	64.5	64.7
S346K/K347Y/K362H	E	K	Y	H	82.9	78.8
E314A/S346K/K347Y/K362H	A	K	Y	H	76.2	75.0
S346I/K347D/K362H	E	I	D	H	88.5	83.1
E314A/S346I/K347D/K362H	A	I	D	H	98.6	90.6

^aResidual enzyme activity by inhibition at 3 mM ATP.

^busing NAD⁺ or NADP⁺ as the cofactor.