Figure 5. Both VP15 and PmFKBP46 present in the nucleus of WSSV-infected hemocytes.
Hemocytes from normal and WSSV-infected shrimp (72 h p.i.) were analyzed by indirect immunofluoresence microscopy. In non-infected hemocytes, PmFKBP46 was visualized (green signal) with a mouse anti-PmFKBP46 antibody and FITC-conjugated goat anti-mouse IgG antibody. In WSSV-infected hemocytes, VP15 was visualized (red signal) with a rabbit anti-VP15 antibody and Cy3-conjugated goat anti-rabbit IgG antibody. Nuclei were visualized by counterstaining with TO-PRO-3 iodide (blue).