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. 2011 Sep 29;6(9):e25420. doi: 10.1371/journal.pone.0025420

Table 1. List of primers used in this study.

Gene Primer sequence (5′-3′) RE site Vector Remarks
PmFKBP46 R1: TATTTTATGGTTTTTAGTTC NA NA 5′RACE
R2: AGATTAGAGTGGAGTTGGGTGGG NA NA 5′RACE
R3: GCTCCTCGCTCACCATACGCCA NA NA 5′RACE
R4: GGGTTTTGTTTTCCACTTTCTTTA NA NA 5′RACE
R5: GTTTGCACTTTATTTCCAGGAGTT NA NA 5′RACE
F: TTCCATATGATGTTTTGGGGTTTGTCAC NdeI pET-15b Recombinant protein production
R: CGCGGATCCTTTTATGGTTTTTAGTTC BamHI
VP15 F1: GAGGCCAGTGAATTCATGACAAAATACCCCGAG EcoRI pGBKT7 Yeast two-hybrid assay
R1: CGATGCCCACCCGGGTGTTAACGCCTTGACTTGCG SmaI
F: CGGATCCATGACAAAATACCCCG BamHI pGEX-4T-3 Recombinant protein production
R: AGCGGCCGCAACGCCTTGACTTGCGGGC NotI

RE site, restriction enzyme site.

NA, not applicable.