Table 1. List of primers used in this study.
Gene | Primer sequence (5′-3′) | RE site | Vector | Remarks |
PmFKBP46 | R1: TATTTTATGGTTTTTAGTTC | NA | NA | 5′RACE |
R2: AGATTAGAGTGGAGTTGGGTGGG | NA | NA | 5′RACE | |
R3: GCTCCTCGCTCACCATACGCCA | NA | NA | 5′RACE | |
R4: GGGTTTTGTTTTCCACTTTCTTTA | NA | NA | 5′RACE | |
R5: GTTTGCACTTTATTTCCAGGAGTT | NA | NA | 5′RACE | |
F: TTCCATATGATGTTTTGGGGTTTGTCAC | NdeI | pET-15b | Recombinant protein production | |
R: CGCGGATCCTTTTATGGTTTTTAGTTC | BamHI | |||
VP15 | F1: GAGGCCAGTGAATTCATGACAAAATACCCCGAG | EcoRI | pGBKT7 | Yeast two-hybrid assay |
R1: CGATGCCCACCCGGGTGTTAACGCCTTGACTTGCG | SmaI | |||
F: CGGATCCATGACAAAATACCCCG | BamHI | pGEX-4T-3 | Recombinant protein production | |
R: AGCGGCCGCAACGCCTTGACTTGCGGGC | NotI |
RE site, restriction enzyme site.
NA, not applicable.