Figure 1. Induction of IFN-γ and IL-2 in splenocytes from RSV F peptide immunized HLA-A*0201 transgenic mice upon secondary recall.
Splenocytes harvested on day 30 from HLA-A-Tg B6 mice primed and boosted at 20 days interval intranasally with 107 pfu of rAd-F0 (□) or 104 pfu of RSV-B1 (▪)were restimulated with 2 µg of the individual RSV F peptide or 10 µg/ml Con A for 5 days in the presence of murine IL-2 . After stimulation, 5×105 splenocytes were seeded to anti-IFN-γ (A) or anti-IL-2 (B) capture antibody coated ELISPOT plates for 2 days for ELISPOT assay as described in Materials and Methods. Cytokine-positive immunospots were developed and the results are expressed as the number of immunospots +/−2 standard deviations for each experimental group. Data is representative of results derived from two independent experiments, each with five mice per group.