Table 1. Characteristics of HLA-A*0201-restricted epitopes of peptide spanning RSV F protein*.
| No. | Sequence | T2-binding Ratio of MFI | IFN-γ ELISPOT | IL-2 ELISPOT | ||
| rAd-F0 | RSV | rAd-F0 | RSV | |||
| N | RPQPRGRRQPIPKARQPEGRL | 1.00 | N/a** | N/a | N/a | N/a |
| EBV | GLCTLVAML | 1.96 | N/a | N/a | N/a | N/a |
| 1 | TLLLWVLLL | 1.19 | 33.5 | 69.5 | 85.5 | 39.5 |
| 2 | LLWVLLLWV | 0.97 | 46.5 | 46.5 | 34.5 | 42 |
| 3 | AITTILAAV | 1.71 | 68.5 | 119.5 | 9 | 31 |
| 4 | ELDKYKNAV | 0.94 | 88.5 | 34.5 | 49.5 | 51.5 |
| 5 | FMNYTLNNT | 0.96 | 73 | 12.5 | 38 | 58.5 |
| 6 | FLLGVGSAI | 1.14 | 65 | 61.5 | 29 | 32 |
| 7 | HLEGEVNKI | 0.83 | 68.5 | 81 | 68 | 33 |
| 8 | ALLSTNKAV | 1.50 | 36 | 86 | 8.5 | 43 |
| 9 | LLSTNKAVV | 1.53 | 27.5 | 1 | 5 | 2 |
| 10 | STNKAVVSL | 0.91 | 43 | 9.5 | 0 | 39.5 |
| 11 | SLSNGVSVL | 1.06 | 68.5 | 200 | 51.5 | 56.5 |
| 12 | VLTSKVLDL | 0.99 | 95.5 | 116.5 | 44 | 36 |
| 13 | KVLDLKNYI | 1.26 | 200 | 103.5 | 85 | 35.5 |
| 14 | YMLTNSELL | 1.71 | 200 | 97 | 200 | 34.5 |
| 15 | KLMSNNVQI | 1.51 | 39 | 0 | 37 | 59.5 |
| 16 | LMSNNVQIV | 1.60 | 54 | 28 | 52 | 50.5 |
| 17 | KIMTSKTDV | 0.95 | 24.5 | 2.5 | 24.5 | 6.5 |
| 18 | SVGNTLYYV | 1.29 | 115 | 92.5 | 0 | 42 |
| 19 | KINQSLAFI | 1.08 | 200 | 7.5 | 52 | 36.5 |
| 20 | IMITTIIIV | 1.03 | 200 | 9 | 32 | 24.5 |
| 21 | IIIVIIVIL | 0.88 | 16.5 | 80 | 2.5 | 21 |
| 22 | VIIVILLSL | 0.97 | 88.5 | 49 | 21.5 | 55 |
| 23 | VILLSLIAV | 1.51 | 130.5 | 87.5 | 34.5 | 43.5 |
| 24 | LLSLIAVGL | 1.71 | 84.5 | 0 | 5 | 42 |
| 25 | SLIAVGLLL | 1.42 | 41 | 96 | 31 | 44.5 |
*Twenty-five HLA-A*0201-restricted 9-mer synthetic peptides from F protein of RSV-B1 strain were tested their binding ability with HLA-A*0201 by T2-stabilization assay, which has been described in the Materials and Methods. The study is a representative of results derived from two independent experiments, each with five mice per group. Results of IFN-γ and IL-2 ELISPOT assays have been described in the legend of figure 1.
**N/a, not assayed.