Table 2. The frequencies of CD11c+ DCs and co-stimulatory molecule expression in wild-type mice given nasal TNP-LPS with or without nCTa.
| Tissue | Nasal Adjuvant | % of total lymphocytes | % of CD11c+ DCs | |||
| CD11ca | CD40b | CD80b | CD86b | MHC IIb | ||
| SMGs | nCT | 1.8±0.6 | 0.5±0.1 | * 2.9±0.5 | *20.9±4.9 | 50.2±8.8 |
| None | 2.0±0.4 | 0.2±0.0 | 0.7±0.2 | 11.3±2.2 | 46.3±10.9 | |
| NPs | nCT | 5.2±2.1 | 3.1±0.8 | * 15.2±2.1 | *7.9±1.3 | 18.0±4.2 |
| None | 5.6±2.6 | 3.4±1.1 | 5.2±0.7 | 3.7±1.1 | 16.9±3.7 | |
| NALT | nCT | 2.2±0.5 | * 7.7±1.5 | * 18.2±3.2 | *17.2±2.8 | 50.6±12.2 |
| None | 2.1±0.7 | 3.7±1.1 | 5.5±1.9 | 7.4±2.1 | 48.1±9.9 | |
a
Mononuclear cells from SMGs, NPs and NALT were isolated five days after immunization with TNP-LPS plus nCT or TNP-LPS alone, and were stained with FITC-conjugated anti-CD11c mAb.
b
Mononuclear cells were stained with FITC-conjugated anti-CD11c and PE-labeled anti-CD40, -CD80, -CD-86 or -MHC class II mAbs and were then subjected to flow cytometry analysis by FACSCalibur®.
*p<0.05 compared with immunized mice given TNP-LPS alone.