Table 3. Induction of IgA CSR on B-1 B cells co-cultured with DCs plus cytokines^a.

Nasal Immunization with	DCs from (4×105 cells/ml)	% of IgA+ cells on B-1 B cells
		IL-5 (100 ng/ml)	TGF-β1 (1 ng/ml)	IL-5 (100 ng/ml)+TGF-β1 (1 ng/ml)
nCT	SMGs	0.5±0.2	0.2±0.2	0.8±0.3
	NPs	0.4±0.2	0.2±0.3	0.7±0.2
	CLNs	0.2±0.3	0.0±0.2	0.5±0.2
TNP-LPS	SMGs	0.4±0.4	0.2±0.8	1.9±0.8
	NPs	0.3±0.2	0.3±0.2	1.2±0.4
	CLNs	0.4±0.5	0.2±2.6	0.9±0.3
TNP-LPS + nCT	SMGs	0.5±0.1	0.3±0.1	* , #4.8±0.5
	NPs	0.4±0.2	0.4±0.2	* , #2.7±0.7
	CLNs	0.4±0.3	0.4±0.1	* , #2.4±0.7

^aIgA negative B cells from SMGs, NPs and CLNs from the peritoneal cavity cells were co-cultured with DCs plus IL-5, TGF-β₁, or both IL-5 and TGF-β₁ for five days, and were stained with FITC-conjugated anti-IgA mAb and were then subjected to flow cytometry analysis by FACSCalibur®.

*p<0.05 compared with immunized mice given TNP-LPS alone.

^# p<0.05 compared with immunized mice given nCT alone.