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. 2011 Sep 29;6(9):e25731. doi: 10.1371/journal.pone.0025731

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Renner et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A–C) Examples of MSD plots of projected trajectories (A) and their corresponding MSD_1Dtransv (B) and MSD_1Dlong (C) for the indicated cylinder diameters. Trajectories were simulated with a diffusivity of 1 µm²/s. Inset in A: the projection of each simulated trajectory was decomposed into two components: parallel and perpendicular to the cylinder axis. These components were used to calculate the longitudinal (MSD_1Dlong) and transversal (MSD_1Dtransv) MSD. D) Effect of cylinder diameter on the values of D_actual, D_proj or D calculated on MSD_1Dtransv (D_1Dlong), for trajectories simulated with a diffusivity of 1 µm²/s (mean ± SD, n = 50).