Table 2. Interactional residues of ligninolytic enzymes with lignin.
Enzyme-lignin complexa | Hb | Ph | Ar | HH | AA |
Lac-lignin | ARG157, ARG161, ASN336, GLN499, GLU460, GLU496, GLY462, HIS55, PHE344, SER113 | ALA80, ARG157, GLN499, GLU460, LEU112, LEU459, LEU58, PHE81, PRO346, SER113 | PHE81, PHE344, PHE450 | ARG157, GLN499, GLU460, LEU459, LEU58, PHE344, PRO346, THR345, TYR491 | GLU460, SER113 |
LiP-lignin | ALA180, ARG43, ASN182, GLU40, HIS176, HIS39, HIS47, ILE338, PRO145, PRO83 | ALA175, ALA179, ALA180, ARG43, ASP183, GLU40, HIS176, HIS39, ILE235, ILE338, ILE42, ILE85, PHE193, PRO147, VAL181, VAL184, VAL90 | PHE193 | ALA179, ALA180, ALA36, ARG43, ASN182, HIS176, HIS39, ILE235, ILE338, ILE85, PRO147, PRO340, VAL184 | GLU146 |
MnP-lignin | ARG177, ARG42, ASP179, ASP241, ASP242, GLU143, GLU35, GLU39, HIS173, HIS38, HIS46, SER172, SER241 | ALA176, ALA178, ARG42, ASP179, HIS46, ILE41, LEU176, LEU239, LYS180, PHE190, PHE45, PRO142, PRO144, VAL175, VAL181 | HIS173, PHE190 | ALA178, ALA79, ARG127, ARG42, GLU39, HIS173, HIS38, HIS46, ILE151, LEU170, LEU239, PHE45, PRO142, PRO144, VAL181 | ARG177 |
Refers to the best docking complex.
LPC/CSU server was used to analyze ligand–protein contacts, including hydrogen bonding (Hb) contact, hydrophobic (Ph) contact, aromatic-aromatic (Ar) contact, hydrophilic-hydrophobic (HH) contact, and acceptor-acceptor (AA) contact.