Figure 5. Plot of relative binding affinity (ΔΔG) against relative decay rate for high-temperature mutations.

Increased stability is associated with relative binding affinities less than zero and decay rates less than one. Error bars show the 95% confidence interval obtained by performing multiple independent experiments and simulations. Most data points lie in the lower left quadrant or on its boundary suggesting qualitative agreement that most mutations are stabilizing. Both methods also concur that F182 is an outlier that destabilizes the capsid.