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. 2011 Sep 29;7(9):e1002297. doi: 10.1371/journal.pgen.1002297

Figure 1. [PSI] strains.

Figure 1

(A) Collection of [PSI] strains. The genetic background is indicated on the left. [PSI] strains are indicated on top. (B) Strain typing by GFP labeling. The strain type is indicated on top. GFP fusion constructs are indicated on the left. Three types of labeling are observed: the 47k strain exhibits particulate labeling by Sup(1-61)-GFP and Sup(1-61)(G20D)-GFP, but diffused GFP fluorescence with Sup(1-61)(Q23P)-GFP; the 21ℓ strain exhibits particulate labeling by all three GFP fusion constructs; 21s, 21w, 28s, 28w, 47s and 47w are only labeled by Sup(1-61)-GFP. (C) Strain typing by colony color changes. A set of yeast centromere-based plasmids (YCp33) encoding the wild type Sup35 protein and single mutants (indicated on top of each panel) is introduced into yeast bearing different [PSI] strains (indicated on the left). Each [PSI] strain gives a distinctive color pattern.