Table 1. Nuclear ribosomal DNA and mitochondrial DNA marker sequences obtained from populations of Lymnaea schirazensis and respective GenBank accession numbers.
DNA marker | Populations | Haplotype No. | Sequence length (nucleotide No.) | GC or AT content (%) | Accession No. |
rDNA 18S | all populations studied | L.schir-18S-H1 | 1852 bp | GC 51.4 | FR772291 |
rDNA ITS-2 | Iran, Egypt, Spain, the Dominican Republic, Venezuela, and one population from Peru (Baños del Inca) | L.schir-H1 | 436 bp | GC 53.9 | JF272601 |
Mexico, Ecuador and one population from Peru (Rio Lurin) | L.schir-H2 | 444 bp | GC 53.8 | JF272602 | |
rDNA ITS-1 | Iran, Egypt, Spain, the Dominican Republic and Venezuela | L. schir -HA | 531 bp | GC 56.1 | JF272603 |
Mexico, Ecuador and Peru | L. schir -HB | 533 bp | GC 55.9 | JF272604 | |
mtDNA 16S rRNA | all populations except one | L.schir-16S-HA | 421 bp | AT 69.6 | JF272605 |
one population from the Dominican Republic (Constanza) | L.schir-16S-HB | 421 bp | AT 69.6 | JF272606 | |
mtDNA cox1 | Iran, Egypt, Spain, the Dominican Republic, Mexico (populations from Los Molinos, Escuela A. Obrego in La Trinidad Tepango, and Jiutepec), Venezuela, Ecuador (population from Machachi) and Peru (population from Rio Lurin) | L.schir-cox1-Ha | 672 bp | AT 69.5 | JF272607 |
one population from Mexico (Xalpatlaco in Atlixco, Puebla) | L.schir-cox1-Hb | 672 bp | AT 69.3 | JF272608 | |
one population from Ecuador (La Buena Esperanza) | L.schir-cox1-Hc | 672 bp | AT 69.6 | JF272609 | |
one population from Peru (Baños del Inca) | L.schir-cox1-Hd | 672 bp | AT 69.1 | JF272610 |
Nuclear rDNA genes and spacers concern complete sequences and definitive haplotype numbers; mtDNA genes only sequence fragments and provisional haplotype numbers. H = haplotype.