Table 2. Sequence differences detected in pairwise comparisons of ITS-2 and ITS-1 between Lymnaea schirazensis and the most morphologically similar species Galba truncatula.

	Alignment length	Total differences		Substitutions				Insertions+deletions
				Transitions		Transversions
Compared species	No. of bp	No.	%	No.	%	No.	%	No.	%
ITS-2:
L. schirazensis H1 vs. G. truncatula H1	477	139	29.14	19	3.98	11	2.31	109	22.83
L. schirazensis H1 vs. G. truncatula H2	477	138	28.93	18	3.77	11	2.31	109	22.83
L. schirazensis H1 vs. G. truncatula H3	477	139	29.14	19	3.98	11	2.31	109	22.83
L. schirazensis H2 vs. G. truncatula H1	469	131	27.93	19	4.05	11	2.34	101	21.53
L. schirazensis H2 vs. G. truncatula H2	469	130	27.72	18	3.84	11	2.34	101	21.53
L. schirazensis H2 vs. G. truncatula H3	469	131	27.93	19	4.05	11	2.34	101	21.53
ITS-1:
L. schirazensis HA vs. G. truncatula HA	560	136	24.28	31	5.53	22	3.93	83	14.82
L. schirazensis HA vs. G. truncatula HB	560	137	24.46	32	5.71	22	3.93	83	14.82
L. schirazensis HA vs. G. truncatula HC	560	138	24.64	32	5.71	23	4.11	83	14.82
L. schirazensis HB vs. G. truncatula HA	558	134	24.01	32	5.73	21	3.76	81	14.52
L. schirazensis HB vs. G. truncatula HB	558	135	24.19	33	5.91	21	3.76	81	14.52
L. schirazensis HB vs. G. truncatula HC	558	136	24.37	33	5.91	22	3.94	81	14.52