Fig. 1. Visual stimulation induces histone mark modifications on specific CRE loci close to miR132 coding sequence and activates mature and primary miR132 expression.
a) Ac(Lys9-14)H3 ChIP showed visually-induced H3 acetylation at the CRE loci upstream of miR132, miR212 and fos. * p<0.05.
b) p(Ser10)Ac(Lys14)H3 ChIP showed visually-induced H3 phosphoacetylation at the CRE sequences upstream of miR132 and fos, but not miR212.
c) Me2(Lys4)H3 ChIP showed visually-induced H3 dimethylation at the CRE sequence upstream of miR132 and fos, but not miR212.
d) Developmental expression of pri-miR132 and miR132 in the visual cortex of STR and DRB mice. Pri-miR132 (left) and miR132 (right) expression significantly increased with age in STR but not in DRB mice. * represents significant differences between STR and DRB.
e) DR3d decreased pri-miR132 (left) and miR132 (right) in visual cortex respect to age matched mice (P27 STR).
f) Visual stimulation after DR3d increased pri-miR132 (left) and miR132 (right) levels. Data are reported as mean ± SEM.