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. Author manuscript; available in PMC: 2012 Sep 27.
Published in final edited form as: ACS Nano. 2011 Aug 17;5(9):7555–7564. doi: 10.1021/nn202554t

Figure 2.

Figure 2

Fluorescence images of a phospholipid vesicle microarray. (a) A microarray uniformly covered with Rho-PE-labeled egg PC vesicles before applying the PDMS squeegee. (b) A microarray after applying the squeegee showing 1 μm-diameter wells with 3 μm periodicity filled with vesicles. (c) Distribution of average fluorescence intensity (red) from 5200 microarray wells and the distribution of surface area (black) of vesicles extruded 21 passes through a 200 nm polycarbonate filter determined by dynamic light scattering. (d) Fluorescence image of the microarray in (b) 2 minutes after photobleaching a 25 μm-diameter area showing no fluorescence recovery. This indicates the material in the wells is isolated and that a supported lipid bilayer does not form spontaneously. The scale bars in a, b and d are 25 μm.