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. Author manuscript; available in PMC: 2012 Sep 23.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Aug 27;413(2):383–388. doi: 10.1016/j.bbrc.2011.08.111

Fig. 1.

Fig. 1

Gemcitabine induces a 110 kD RRM1 band. (A) HEK293 cells were treated with 10% FBS, 50 J/m2 UV, 1 μM gemcitabine (Gem); 100 μM etoposide (VP16), or 300 μM H2O2. RRM1 was immunoprecipitated (IP) and Western blotted (WB) with the same antibody. (B) Four cell lines were treated with 1 μM gemcitabine. (C) HEK293 cells were treated with 1 μM of gemcitabine. Immunoprecipitated RRM1 was separated in a SDS-PAGE gel and stained with Coomassie blue. Normal goat IgG was used as a control.