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. Author manuscript; available in PMC: 2012 Oct 4.
Published in final edited form as: Biochemistry. 2011 Sep 6;50(39):8270–8280. doi: 10.1021/bi200423t

Figure 6.

Figure 6

DrRecA formed more gaps and much shorter nucleoprotein filaments than EcRecA as visualized by electron microscopy. Reactions were carried out as described under Materials and Methods. (A) A representative circular dsDNA bound by DrRecA containing 6 gaps and 6 nucleoprotein filaments. (B) Quantification of 27 circular dsDNA molecules bound by DrRecA shows that DrRecA forms a total of 368 short filaments within that population, most of which are very short. (C) A representative circular dsDNA bound by EcRecA containing 3 gaps and 3 nucleoprotein filaments. (D) Quantification of 28 circular dsDNA molecule bound by EcRecA shows that EcRecA forms 113 nucleoprotein filaments that vary from short to very long, up to 2599 nm.