Lower tibia and femur trabecular bone volume in 12-week-old male KO mice. (A) The tibias of 4-, 8-, and 12-week-old male WT and KO mice were analyzed by µCT. Trabecular bone volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), connectivity density (Conn.D), and trabecular separation (Tb.Sp) were determined. (B) Tibia cortical bone analysis of 12-week-old male mice revealed that KO mice had a slightly increased endosteal perimeter. Cortical cross-sectional area (Cort.CSA), cortical thickness (Cort.Th), periosteal perimeter (Perios.perim), and endosteal perimeter (Endos.perim) were determined. (C) Static bone histomorphometric analysis demonstrated reduced femoral, but not spine, trabecular bone volume in 12-week-old KO male mice. (D) Representative tibias from WT and KO mice stained with H&E/orange G (*p ≤ 0.05; **p ≤ 0.01).