Sequence and expression analysis of the ppp1r14al gene. (A) Phylogenetic tree demonstrating that zebrafish ppp1r14al groups with other ppp1r14a genes, not with ppp1r14b genes. (B-E) ppp1r14al expression pattern at 9hpf (B), 11hpf (C) and 20hpf (D, E). Arrows indicate cranial placodes (D, E) and arrowheads indicate staining in ventral mesoderm (C) and in the lateral neural tube (D, E). E is a double in situ hybridization with krox20 detected in red. Embryos in B-E are shown as flat mounts in dorsal view with anterior to the top.