Disruption of ppp1r14al leads to loss of fgf3 expression. (A-N) Control (A, C, E, G, I, K, M) and ppp1r14al MO-injected (B, D, F, H, J, L, N) embryos were assayed for expression of fgf3 at 10.5hpf (A, B), DUSP2 at 10.5 hpf (C, D), hoxb1a at 11.5 hpf (E, F) krox20 at 10.5hpf (G, H) and at 12hpf (I, J), pea3 at 12hpf (K, L) and valentino at 12.5hpf (M, N). (O-R) Analysis of fgf3 expression in control (O), ppp1r14al MO-injected (P), ppp1r14al MO + ppp1r14al mRNA injected (Q; note that mRNA is mutated to resist MO effect) reveals rescue of the ppp1r14al MO phenotype at 10.5hpf. Actual data is given in R. Phenotypes were scored as severe (similar to panel P), or mild (similar to panel B). Embryos in A-Q are shown as flat mounts in dorsal view with anterior to the top.