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Combined loss of ppp1r14al and fgf8 function leads to disruption of the r4 signaling center. Uninjected (A, E, I), fgf8 MO-injected (B, F, J), ppp1r14al MO-injected (C, G, K) or fgf8 + ppp1r14al MO-injected embryos (D, H, L) were assayed for expression of krox20 (A-D), pea3 (E-H) or valentino (I-L) by in situ hybridization. All embryos are dorsal views at 11hpf with anterior to the left.
