Fig. 3.

Neuroprotection evoked by delayed application of the single dose of biphalin (BIPH) in vitro in organotypic hippocampal culture (OHC) challenged with NMDA (100 μM) for 3 h. Quantitative analysis of cell death of OHC, 24 h after glutamatergic stress and 0.1 μM BIPH application at 0.5, 1 or 1.5 h after NMDA. The results are expressed as the mean ± SD (n = 16–24) of propidium iodide (PI) positive cells. Values are considered significant where *P < 0.05 or **P < 0.01 versus NMDA treated cultures or #P < 0.05 versus control