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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Bone Miner Res. 2011 Oct;26(10):2498–2510. doi: 10.1002/jbmr.443

Figure 4.

Figure 4

Enhanced hypertrophic differentiation at late gestation in Smad6−/− mice. (A,B) Shorter diaphyses are evident in E18.5 Smad6−/− tibias, as demarcated by black bars. (C,D) Higher magnification of proximal tibias at E18.5. (E) Immunofluorescence staining of adjacent sections of E18.5 distal femurs for the hypertrophic differentiation marker, type X collagen, and terminal differentiation markers, osteopontin (OPN) and MMP-13. The hypertrophic zone is demarcated by white bars. The region of expression is demarcated by blue bars. No detectable staining was observed in negative controls, from which primary antibodies were omitted. (F) Immunofluorescence staining of tibial growth plates for type II collagen shows enhanced collagen production at P0. No detectable staining was observed in negative controls. (G) Semiquantitative RT-PCR analysis of RNA isolated from WT and Smad6−/− primary chondrocytes cultured in chondrogenic media for 7 days.