Figure 3.

At a constant level of cell–cell adhesion, cell population spreading on poly(DTE-co-PEG carbonate) substrates depends on the adhesivity of the substratum. Single cell suspensions of all L cell types, exemplified here by LN5 (a–d) and LR1 (e–h) were allowed to attach for 24 h to polymeric substrata containing 0–5% PEG. Note cadherin-specific differences in culture morphology. In all cases, cell population spreading was markedly reduced by increased PEG content of the substratum (top to bottom). Cells seeded on the substratum of highest PEG content tended to aggregate on the substratum, from which the aggregates were easily removed by gentle agitation. (Scale bar = 100 μm.)