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Serum-derived proteins mediate cell aggregate spreading. PKH2 fluorescence-labeled cell aggregates of low cohesivity (LN2 cells, 1.9 dyne/cm) were deposited and cultured for 24 h on a poly(DTE-carbonate)-coated surface in medium containing 10% FBS (Left) or TCM (TM) serum substitute (Right). Cells emigrated rapidly in the presence of serum but not at all in its absence.
