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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Protein Expr Purif. 2011 Jul 14;80(1):8–16. doi: 10.1016/j.pep.2011.06.013

Fig. 4.

Fig. 4

Purification of the construct of MBP-tevs-LXXLL-E6 (A-B) and MBP -LXXLL-tevs-E6 (C-D) constructs. (A and C) 15% SDS-PAGE gels for the two purifications. Lane 1: total cell lysate; lane 2: eluate from amylose resin before overnight ultracentrifugation; lane 3: eluate from amylose resin before overnight ultracentrifugation; lane 4: TEV protease digest; lane 5: pooled gel-filtration fractions at the elution volume expected for the E6/paxillin complex. (B and D) Gel filtration profiles of LXXLL-E6 and E6/LXXLL samples after separation from the MBP. Peaks corresponding to MBP-tevs or MBP-LXXLL-tevs and to E6/paxillin complexes are indicated. See also legend of Fig. 1.