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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Hepatol. 2011 May 18;56(1):192–198. doi: 10.1016/j.jhep.2011.03.019

Fig. 1. Effects of IRE1α and ASK1 depletion on JNK activation.

Fig. 1

Hepa1c1c7 cells were transfected with non-silencing control or siRNA specific for ASK1 and incubated with 1 μM thapsigargin for 1 h (A) or with 0.5 mM palmitic acid for 6 h (B). The efficacy of ASK1 depletion and the expression and phosphorylation of JNK was examined by immunoblot analysis. Hepa1c1c7 cells were transfected with non-silencing control or siRNA specific for IRE1α and incubated with 1 μM thapsigargin for 1 h (C) or with 0.5 mM palmitate for 6 h (D). The expression of IRE1α and the expression and phosphorylation of JNK was examined by immunoblot analysis. Hepa1c1c7 cells were transfected with non-silencing control or siRNA specific for IRE1α and ASK1 and incubated with 1 μM thapsigargin for 1 h (E) or with 0.5 mM palmitate for 6 h (F). The expression of IRE1α and ASK1 and the expression and phosphorylation of JNK was examined by immunoblot analysis.