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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Hepatol. 2011 May 18;56(1):192–198. doi: 10.1016/j.jhep.2011.03.019

Fig. 2. Activation of Cdc42 and Rac1 SFA.

Fig. 2

AML12 cells were incubated with 0.5 mM palmitate for the indicated time points. Cdc42 (A) and Rac1 (B) activation was determined by binding to GST-PAK PBD and subsequent immunoblot analysis. (C) AML12 cells were incubated with palmitate for 30 min. Cells were fractionated and the membrane fraction was analyzed for translocation of Cdc42 and Rac1 by immunoblot analysis. (D) AML12 cells were incubated with BSA (B), oleate (O) or palmitate (P). Cdc42 activation was determined by binding to GST-PAK PBD and subsequent immunoblot analysis.