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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Dev Biol. 2011 Aug 18;358(2):331–343. doi: 10.1016/j.ydbio.2011.08.008

Figure 3. Cultured Tgfbr3−/− epicardial cells can undergo EMT and smooth muscle differentiation.

Figure 3

Tgfbr3+/+ and Tgfbr3−/− cells were incubated with vehicle, 250 pM TGFβ1 or TGFβ2 for 72 hours. (A) Immunohistochemistry: Tgfbr3+/+ (top) and Tgfbr3−/− (bottom) cells incubated with vehicle localize the epithelial marker, ZO-1 to cell margins. TGFβ1 or TGFβ2 induces loss of cell-cell contact and loss of ZO-1 from cell margins. (B) Tgfbr3+/+ (top) and Tgfbr3−/− (bottom) cells incubated with vehicle do not show SM22α in stress fibers. TGFβ1 or TGFβ2 induces SM22α expression in stress fibers in both genotypes. (C and D) Induction of the smooth muscle markers, SM22α, SMαA, and Calponin was evaluated using qRT-PCR analysis (n=3; *p<0.05)