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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Dev Biol. 2011 Aug 18;358(2):331–343. doi: 10.1016/j.ydbio.2011.08.008

Figure 5. The cytoplasmic domain of TGFβR3 and GIPC are required for invasion.

Figure 5

Invasion was analyzed as described in Figure 2D for all panels (A) TGFβR3 contains a 43 amino acid cytoplasmic domain whose C-terminal 3 amino acids, STA, bind GIPC. (B) Tgfbr3−/− epicardial cells infected with adenovirus co-expressing GFP and either FL, CYTO or Δ3 receptor and incubated with vehicle, 250 pM TGFβ2, 10ng/ml FGF-2 or 300 μg/ml HMW-HA. (C) Tgfbr3+/+ or Tgfbr3−/− cells infected with adenovirus co-expressing GFP and GIPC1. (D) Tgfbr3+/+ cells transfected with siRNA to GIPC1 and incubated with vehicle, 250 pM TGFβ2, 10ng/ml FGF-2 or 300 μg/ml HMW-HA. (n=3, replicates of 6,*=p<0.05). (E) Tgfbr3−/− cells infected with adenovirus expressing GFP or FL receptor, transfected with siRNA to GIPC1, and incubated with vehicle, 250 pM TGFβ2, 10ng/ml FGF-2, or 300 μg/ml HMW-HA. (n=3, replicates of 4). *= [GFP+scramble+ligand] vs. [FL+scramble+ligand];#[FL+scramble+ TGFβ2] vs. [FL+GIPCsiRNA+TGFβ2];$[FL+scramble+FGF2] vs. [FL+GIPCsiRNA+ FGF2]; & [FL+scramble+HMW-HA] vs. [FL+GIPCsiRNA+ HMW-HA]; p<0.05