. Author manuscript; available in PMC: 2012 Nov 1.

Published in final edited form as: Protein Expr Purif. 2011 Jul 23;80(1):22–27. doi: 10.1016/j.pep.2011.07.005

Table 1.

Protein recovery levels for G117H/E197Q, W541H₆Δ.

	DMEM-F12 1:1		CD-CHO
	Units^a	P. D.^b	Units	P. D.
Clarified media	9.1	100	2.7	100
Flow through + Wash	0.1	10	0.3	10
Elution	8.7	95	2.3	86

G117H/E197Q, W541H₆Δ was expressed in two different media supplemented with FBS and Gln, and purified as described in SI above. The units and percent distribution (P. D.) were recorded for each step.

A unit is defined as the amount of enzyme required to hydrolyze 1 μmole BTC min⁻¹ at room temperature in PBS pH 7.4 containing 1 mM BTC.

P. D. was calculated by dividing the total Units in a given sample by the total Units in the clarified media and multiplying by 100.