Table 1.
DMEM-F12 1:1 | CD-CHO | |||
---|---|---|---|---|
Unitsa | P. D.b | Units | P. D. | |
Clarified media | 9.1 | 100 | 2.7 | 100 |
Flow through + Wash | 0.1 | 10 | 0.3 | 10 |
Elution | 8.7 | 95 | 2.3 | 86 |
G117H/E197Q, W541H6Δ was expressed in two different media supplemented with FBS and Gln, and purified as described in SI above. The units and percent distribution (P. D.) were recorded for each step.
A unit is defined as the amount of enzyme required to hydrolyze 1 μmole BTC min−1 at room temperature in PBS pH 7.4 containing 1 mM BTC.
P. D. was calculated by dividing the total Units in a given sample by the total Units in the clarified media and multiplying by 100.