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. Author manuscript; available in PMC: 2012 Dec 9.
Published in final edited form as: J Chromatogr A. 2011 May 27;1218(49):8791–8798. doi: 10.1016/j.chroma.2011.05.067

Figure 6.

Figure 6

Effect on the ATPase activity of an Hsp90α(CT) column of the addition of either the Hsp90-binding drug, geldanamycin, to the mobile phase or from diamide-mediated S-glutathionylation of the immobilized Hsp90. Calculation of the ADP/ATP ratio represents the AUC (expressed as ion abundance) of the peak produced by mass spectral analysis of ATP and ADP. Bars represent the mean ± S.D. of three independently conducted experiments.