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Immunolocalization of Type I collagen in silk scaffolds. The scaffolds show positive immunohistochemical staining for Type I collagen (A–I) showing ample extracellular matrix formation. The initial seeding densities were 25 million cells/ml (A–C), 50 million cells/ml (D–F) and 100 million cells/ml (G–I). Yellow box indicates region of higher magnification. Scale bars represent 100μm.
