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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Pain. 2011 Jul 6;152(10):2267–2276. doi: 10.1016/j.pain.2011.06.002

Figure 4.

Figure 4

Targeting peripheral 5HT receptors attenuates calcium accumulation in capsaicin-sensitive cultured TG neurons. Cultures were pretreated with 40 nM sumatriptan, 10 nM ketanserin, 10 nM granisetron, a combination of all 3 drugs or vehicle for 5 minutes followed by treatment with 1 µM 5HT for 3 min (A). Capsaicin-sensitive neurons were identified by stimulation with capsaicin (30 nM) following 5HT treatment. Also shown is the effect of drug treatment on capsaicin-evoked calcium accumulation (B) and pretreatment with GR 55562 prior to sumatriptan and 5HT treatment versus vehicle in capsaicin-sensitive cells (C) reported as change from baseline (Δ). Representative time courses of the effect of 5HT followed by capsaicin on calcium influx following each drug treatment (D–F) reported as actual ratio. Data is shown as mean±SEM change from baseline (n=53–73 per group). Asterisks denote significant reduction at p<0.05 compared to 5HT treatment alone.