Table 1.
Riboprobes for 5HT receptor subtype mRNA localization using in situ hybridization.
| Subtype | Primers | Position (bp) |
Product Size (bp) |
NCBI Accession No. |
|---|---|---|---|---|
| 5HT1B | Forward 5’-TGGAGGAGCAGGGTATTCAG-3’ | 2-21 | 1043 | NM_022225.1 |
| Reverse 5’-GTCAAAAATGGCCATGTGAA-3’ | 1044-1025 | |||
| Forward 5’-TGTTAGCGCTCATCACCTTG-3’ | 152-171 | 443 | ||
| Reverse 5’-GTTCACAAAGCAGTCCAGCA-3’ | 594-575 | |||
| 5HT1D | Forward 5’-CTGCCAAACCAGTCCCTAGA-3’ | 7-26 | 1108 | NM_012852.1 |
| Reverse 5’-TTCGGAAATGGACGACTCTC-3’ | 1114-1095 | |||
| Forward 5’-TCTGTGTCATCGCTCTGGAC-3’ | 377-396 | 493 | ||
| Reverse 5’-GCAGAGATCCTCTTGCGTTC-3’ | 869-850 | |||
| 5HT2A | Forward 5’-AGCTCTGTGCGATCTGGATT-3’ | 496-515 | 1066 | NM_017254.1 |
| Reverse 5’-TTGTTGCAGCCTCCTTATCC-3 | 1561-1542 | |||
| Forward 5’-GCGATCTGGATTTACCTGGA-3’ | 504-523 | 596 | ||
| Reverse 5’-ACGGCCATGATATTGGTGAT-3’ | 1099-1080 | |||
| 5HT2C | Forward 5’-AACTGGCCAGCACTTTCAAT-3’ | 163-182 | 1036 | NM_012765.2 |
| Reverse 5’-TCTGTCGAACAGGAGGCTTT-3’ | 1198-1179 | |||
| Forward 5’-GATGGTGGACGCTTGTTTCAATTCCCGGA-3’ | 124-152 | 612 | ||
| Reverse 5’-TTGACGGCGCAGGACGTAGATCGTTAAGA-3’ | 735-707 | |||
| 5HT3A | Forward 5’-CTTGCTGCCCAGTATCTTCC-3’ | 1091-1110 | 1011 | NM_024394.1 |
| Reverse 5’-GTCTCAGGCACCTAGGCAAG-3’ | 2101-2082 | |||
| Forward 5’-GAGACCATCTTCATTGTGCAGCTGGTGCA-3’ | 904-932 | 415 | ||
| Reverse 5’-ACAGCAGCGTGTCCAGCACATATCCCACC-3’ | 1318-1290 |