Table 3.
Roles of axon outgrowth and branching genes in PLM regrowth.
| Gene name | Mutations | Normalized regrowth | P value | PLM Development | Molecular function | Closest human gene |
|---|---|---|---|---|---|---|
| a. Known axon outgrowth mutants
| ||||||
| unc-53 | e404 | 0.12 ± 0.05 | *** | 100% US | NAV | NAV2 |
| unc-115 | e2225 | 0.28 ± 0.04 | ** | 5% OS, 14% branchless | Actin-binding LIM | ABLIM1 |
| unc-73 | e936(lf), rh40 | 0.31 ± 0.1 | *** | 100% US | GEF | TRIO |
| unc-69 | e587, ju69 | 0.43 ± 0.09 | * | 60% US (e587); Normal (ju69) | Kinesin interactor | FEX2 |
| unc-51 | e369, ky347(lf) | 0.47 ± 0.10 | * | 30% US (ky347) | Atg1 kinase | ULK2 |
| unc-76 | e911 | 0.67 ± 0.12 | ns | 95% US, 80% branchless | Kinesin interactor | SCOC |
| unc-16 | e109 | 0.68 ± 0.09 | ns | 7% OS, 10% branchless | JIP1 scaffold | SPAG9 |
| unc-44 | e362 | 0.77 ± 0.22 | * | 5% OS, 5% US | Ankyrin | ANK1 |
| unc-33 | e204 | 0.79 ± 0.12 | ns | 2% OS, 10% branchless | CRMP/TOAD | DPYSL3 |
|
| ||||||
| b. Other mutants with reduced PLM axon outgrowth or branching defects
| ||||||
| deg-3 | u662 | 0.42 ± 0.07 | *** | 50% short axons | nAChR subunit | CHRNA7 |
| pig-1 | gm144 | 0.55 ± 0.08 | * | 40% US | leucine zipper kinase | MELK |
| wsp-1 | gm324 | 0.83 ± 0.08 | ns | 90% branchless | WASP | WASL |
| ddr-1 | ok874 | 0.74 ± 0.12 | ns | 25% branchless | Discoidin domain receptor | DDR2 |
| hst-2 | ok595 | 0.56 ± 0.10 | * | 11% branchless | Heparan sulfotransferase | HS2ST1 |
| klc-1 | ok2609 | 0.82 ± 0.07 | ns | 10% extra branch | Kinesin light chain | hKLC1B |
| klp-10 | ok704 | 0.85 ± 0.06 | ns | 15% extra branch | Kinesin-like | KIF15 |
| nfi-1 | qa524 | 0.79 ± 0.13 | ns | 50% wavy process | Nuclear factor I | NF1A |
| oig-1 | ok1687 | 0.70 ± 0.10 | ns | 26% extra branch | One Ig Domain | PTPRF? |
| rack-1 | tm2262 | 0.74 ± 0.10 | ns | 10% extra branch | Receptor of C- kinase | GNB2L1 |
| ver-3 | gk227 | 1.28 ± 0.17 | ns | 10% extra branch | VEGFR | FLT1 |
| F09A5.1 | ok2286 | 0.86 ± 0.08 | * | 18% extra branch | Spinster | SPINL |
| vang-1 | ok1142 | 0.78 ± 0.10 | ns | 40% proximal branch | Van Gogh/Strabismus | VANGL1 |
| Y38H8A.4 | ok2793 | 0.73 ± 0.07 | * | 20% branchless | Serine/Threonine kinase | TSSK2 |